지방흡입 Things To Know Before You Buy
지방흡입 Things To Know Before You Buy
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It really is crucial which the submerged growth cultures in inserts reach one hundred% confluence prior to air-lifting. At a hundred% confluence, the cells will protect the surface through the insert forming a whole, uniform monolayer.
Best success are acquired when restricting extended cell publicity to ambient temperature circumstances. Look at holding unused cells inside a humidified incubator with five% CO2 at 37°C when undertaking larger experiments.
Stream cytometry may be used to measure the effectiveness of differentiation protocols With all the STEMdiff™ system, by investigating certain marker expression at Each individual stage of the differentiation approach. It can also be utilized for other applications, which include cell sorting, immunophenotyping, and purity assessment.
Get the job done immediately at this stage to pool wells into a 15 mL tube. Note: For a significant-scale dissociation, utilize a multichannel pipette to pool cells right into a sterile reagent reservoir. Incorporate the pooled wells into a 15 mL tube.
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For greatest results, the full quantity of cargo additional should not exceed ten% of the response volume. Lessening the reaction quantity to below 80 µL may perhaps cause reduce enhancing efficiencies and 줄기세포 지방이식 cell recoveries.
Likely again to the plate, rinse Every effectively with one mL of FACS buffer and transfer the amount to your fifteen mL tube. Take note: Continue to keep cell suspension on ice after transfer on the tube until eventually wanting to operate FACS.
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Certainly, you’ll locate the techniques to conduct an ICC staining on your own epithelial cells cultured at the ALI in this protocol. Here's a listing of antibodies that could be utilized for the characterization of airway cultures:
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Conduct cell counts applying an automatic cell counter. Note: Reduced viability could be optimized with the size of collagenase or ACCUTASE™ 지방흡입 incubation. It can even be optimized by reducing the number of triturations.
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